Identifikasi Molekuler Jamur Pembusuk kayu dari Pohon kanker Meranti Merah (Shorea smithiana) dan Meranti Kuning (Shorea gibbosa)

Abstract

By molecular technique, identification of decay fungi isolated from decayed wood of the tree canker of red meranti (Shorea smithiana) and yellow meranti (Shorea gibbosa) have been carried out. In this molecular identification, the ITS region of rDNA of fungal isolates were amplified using PCR. The DNA sequence for each fungus was aligned together with that of known species in the GenBank database (National Centre for Biotechnology Information) US National Institute of Health Bethesda. By BLAST method and phylogenetic analysis based on the sequences of the ITS region of rDNA, Basidiomycete fungi isolated from decayed wood of red meranti and yellow meranti could be identified. The presence of ascomycete fungi on the decayed wood samples was also detected.