| dc.description.abstract | This study investigated the adjuvant effect of Boesenbergia pandurata (BP), Zingiber zerumbet (ZZ), Solanum
ferox (SF) on protection of tilapia with injection Pseudomonas sp. (Pseumulvacc) vaccination. The extract
concentrations of BP (600 mg/L), ZZ (200 mg/L), and SF (900 mg/L) were combined with the vaccine, ratio
between vaccine and extract was 1:1. Tilapia fish were injected with vaccine mix the extract and challenged at day
7 (d7), 14 (d14), and 21 (d21) post vaccination through injection with A. hydrophila and P. fluorescens (105 CFU/
mL each bacteria). The results shown that the fish with BP+V were found in fin rot at d14 days challenge. The
same symptom was found in ZZ+V at d14 challenge as much 11.11% and 42.86% while, in the vaccine groups
(V), after the challenge, tilapia were found fin rot and darkness color until the last experiment. The BP+V and
SF+ZZ+V groups shown reducing the number of bacteria in the fish body after challenge test on d7, d14, and
d21. The efficacy of Pseumulvacc vaccine has increased after its administration with BP (BP+V) on day 7 and day
14 after challenge (90%) and 100% at the time of challenge test d21. The conclusion is B. pandurata extract was
a promising adjuvant candidate, and the extract is the best plants as an adjuvant that mixed with the vaccine to
against A. hydrophila and P. fluorescens infection. | |
