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dc.contributor.authorDarma, Surya
dc.date.accessioned2022-03-12T12:17:28Z
dc.date.available2022-03-12T12:17:28Z
dc.date.issued2022-03-03
dc.identifier.issn2413-886X
dc.identifier.urihttp://repository.unmul.ac.id/handle/123456789/24305
dc.descriptionThe ISSR profile was used to determine the genetic similarity standard, which was used to construct the dendrogram by the UPGMA method. The first and second major coordinates accounted for 52.5% and 10.0% of the total variation, respectively. Among the cultivars cultivated in the first group, M. alba belongs to the genus Macromorse in the morphological classification of the genus Morus and differs independently from other mulberry species, cultivated M. nigra and wild M. nigra are grouped together. Close correlation with 0.9348 genetic similarity coefficient. Dendrograms obtained with ISSR markers (Figure 3) show similar topology with ISSR markers from other authors, although there are some differences in the placement of some genotypes. From PCA analysis, as in other marker methods, cultured mulberry genotypes are not fully integrated, possibly since different molecular markers are formed due to differences.en_US
dc.description.abstractMulberry (Morus Sp.) is one of the economically important trees cultivated for the tasteful fruits. In Saudi Arabia, Mulberry grown well and spread in different places such as Taif Province (El Shafa region), Eastern region; Al-Ehsaa Province and some southern areas. Nodal explants of Morus nigra were clonally propagated in vitro for plant regeneration. Auxiliary shoot buds have been promoted in Murashige and Scoog (MS) media in a variety of cultural contexts. The largest number of shoots (13.00 ± 0.47) with an average length of 2.00 ± 0.47 cm were initially obtained from a medium containing 2.0 mg / L N6-benzyladin (BA) and 3% sucrose. Recurrent subcultures provided the highest number of seedlings (approximately 29.30) for excavation after the fourth passage. Seedlings were rooted in 1/2 MS medium supplemented with 1.0 mg / 1 indole-3-butyric acid (IBA). Successfully, about 90% of the plantlets acclimatized. Along with determination of the genetic variations between three mulberry genotypes including two cultivated accessions (Morus alba) and one wild genotype (M. nigra) utilizing inter-simple sequence repeat (ISSR) markers. Genetic variation and phylogenetic relationship of mulberry germplasm collection have been studied. All ISSR markers used in this study revealed higher genetic diversity was in the wild species comparing with cultivated species. ISSR matrices reported that the mean genetic similarity coefficient was 0.7677 for all mulberry genotypes. Although some differences have been observed, much similarity has been obtained in dendrogram topology. Cluster analysis of the ISSR using UPGMA software revealed that wild species were genetically distinct. The correlation coefficients of similarity for the ISSR used are statistically important. The Principal Coordinate Analysis (PCA) for ISSR data also supports its UPGMA clustering. The average number of genetic variations recorded in mulberry genotypes was 0.287±0.096. Dendrogram (Un-weighted peer group method analysis) classifies mulberry accessions into two main groups; Admissions collected from western area of Taif, and the other comprised two sub-clusters including one isolate, i.e., M nigra, a collection from Al shafa. Contains access to another sub-cluster southwest regions of Taif, which belong to Morus nigra wild growing. These accessions of mulberry were found to be genetically similar from north and southwest Taif Province. These results have significant implications for improving the mulberry germ plasma characterization, conservation and investigates the genetic diversity among the mulberry species grown in Taif governorate and to establish a micro-propagation system as germplasm conservation to preserve the assets of local mulberry and thus develops an easy and effective method to identify native genotypes in a limited space and time frame.en_US
dc.description.sponsorshipNoneen_US
dc.language.isoenen_US
dc.publisherAcademic Research Publishing Groupen_US
dc.relation.ispartofseriesCertificate of Appreciation;44377 - jac
dc.subjectMorus nigraen_US
dc.subjectin vitro propagationen_US
dc.subjectgenetic distancesen_US
dc.subjectISSR markersen_US
dc.titlePengulas di Journal of Agricultural and Crops (JAC)en_US
dc.title.alternative“Molecular Assessment of Established Clonal Propagated (Morus nigra L.).”en_US
dc.typeOtheren_US


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