| dc.description.abstract | Disease caused by infection with pathogenic bacteria is a problem that often causes losses in fish farming activities around the Mahakam River, Kutai Kartanegara Regency, East Kalimantan Province, Indonesia. Utilization of probiotics is a strategy in disease control in aquaculture activities, especially those related to bacterial infections. Lactic acid bacteria (LAB) originating from fish intestines is a type of probiotic bacteria that has been proven to be able to improve fish health through the use of nutrients, antibacterial properties and increasing their immune response in dealing with pathogenic infections. The potential of local fish as a source of probiotic bacteria needs to be investigated considering the presence of this type of fish is still abundant in the waters of the Mahakam River and surrounding lakes. This study aims to isolated and characterized LAB from the intestines of repang fish Puntioplites waandersi which have potential as probiotic bacteria in vitro.
The sample consisted of ten repang fish with a length of about 15.39±0.65 cm and a weight of about 40.04±6.24 g, derived from the catch of fisherman in the Mahakam River, Kutai Kartanegara Regency, East Kalimantan Province, Indonesia. The tests carried out included isolation of LAB, characterization of isolates, antibacterial activity against pathogenic bacteria Aeromonas hydrophila, Pseudomonas sp. and Edwardsiella ictaluri, antibiotics sensitivity, tolerance to pH, bile salt, NaCl, temperature and enzymatic activity such us amylolytic, proteolytic and lipolytic,
Result of this study showed that five isolates of LAB that have been isolated are Enterococcus sp. (R1 and R2), Lactobacillus sp. (R3), Lactococcus sp. (R4 and R5). The five LAB have strong antibacterial activity againts pathogens (Table 1), some are resistant to antibiotics and have tolerance to pH, bile salts, salinity and temperature, and exhibited amylolytic, proteolytic and lipolytic activities, so these LAB have potential as probiotics.
Table 1. Diameter inhabitation zone (mm) of LAB from intestines
of Puntioplites waandersi against pathogens
Isolates Aeromonas hydrophila Pseudomonas sp. Edwardsiella ictaluri
R1 11,67 10,00 11,33
R2 12,33 13,67 11,00
R3 12,00 11,67 14,00
R4 11,00 12,67 13,00
R5 15,33 12,33 13,33
Control + 17,00 19,00 16,00
Control - 8,00 6,00 7,00 | en_US |
