The Effect of Acute and Chronic Infection-Induced by AvrA Protein of Salmonella typhimurium on Radical Oxygen Species, Phosphatase and Tensin Homolog, and Cellular Homolog Expression During the Development of Colon Cancer
Date
2021-04-23Author
Rachmi, Eva
Satuman, Satuman
Sari, Desi
Tanggo, Eddy
Notobroto, Hari
Sudiana, Ketut
Mubarika, Sofia
Rantam, Fedik
Soemarno, Soemarno
Warsito, Eddy
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Show full item recordAbstract
AIM: The study aimed to analyze the effect of AvrA effector protein of Salmonella typhimurium in inducing colon
cancer through increased of radical oxygen species (ROS), phosphatase and tensin homolog (PTEN), and avian
myelocytomatosis virus oncogene cellular homolog (c-Myc) expression, in mice model of colorectal cancer.
METHODS: This study used Balb/c mice which were divided into four types of groups: Negative control, exposed
to azoxymethane (AOM), treatment with AOM, and AvrA (AOM+AvrA), and treatment with AOM and S. typhimurium
(AOM + S. typhimurium). Each type consists of a 1-week treatment group and a 12-weeks treatment group, with a
final number of eight groups. S. typhimurium-specific protein (AvrA) was isolated and then injected to AOM + AvrA
groups (40 μg/50 μl), intraperitoneally. S. typhimurium was administered orally to AOM + S. typhimurium groups. ROS
production in peripheral blood mononuclear cells was measured by flow cytometry. PTEN and c-Myc expression in
colon tissue were detected through immunohistochemistry.
RESULTS: The study showed that ROS production was higher in the 12-week AOM + S. typhimurium treatment
group compared with other 12-week treatment groups (p < 0.05). AOM + AvrA and AOM + S. typhimurium groups
demonstrated a decrease of PTEN expression and an increase of c-Myc expression in colon tissue, compared to
AOM groups, both in 1-week and 12-weeks treatment (p < 0.05).
CONCLUSION: AvrA effector protein from S. typhimurium increased ROS production and c-Myc expression while
suppressed PTEN expression as markers of colorectal cancer, both in acute and chronic infections
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