Turnitin Report_Method Development and Validation for Lead (Pb) Analysis in Natural Honey from East Kalimantan

Abstract

Analysis method for determining Pb content in natural honey have been developed and validated. Analysis were carried out on one natural honey sample collected from Teririp (Balikpapan) with standard analysis method and three developed analysis methods and their analytical performance were compared. Pb content was determined with atomic absorption spectrometry after dry digestion (method A) or wet digestion (method B (HNO3-H2O2;3:8), C (HNO3-H2O2;2:1) and D (HNO3)). Analytical performance parameter, such as linearity, working range, limit of detection, limit of quantification, precision and accuracy were tested. Content of Pb in natural honey sample which analysed with method A, B, C and D were found at 0.7923  0.0311 g/g, 0.7944  0.0216 g/g, 0.7998  0.0218 g/g and 0.7912  0.0160 g/g respectively. gAnalytical performance of four tested analytical method were still acceptable. Significance testing result showed that there were no significance differences in precision and accuracy between developed analysis method and standard analysis method, except for method D. Precision of method D was significantly better than standard analysis method