Enhanced Extraction of Total Polyphenols Content from Mitragyna Speciosa (Korth.) Havil Leaves using the Natural Deep Eutectic Solvent-based Microwave-assisted Extraction Method

Abstract

Exploration of natural products is highly dependent on separation techniques, mainly solvent selection, one of which is using the green chemistry principle approach. Mitragyna speciosa (Koth.) Havil is an endemic plant of East Kalimantan which traditionally used for the treatment of various diseases. On the other hand, this plant has an addictive effect. The study aims to determine the impact of using natural deep eutectic solvent-based microwave-assisted extraction (NADES-MAE) on total polyphenol content (TPC) extraction from M. speciosa. Natural deep eutectic solvent (NADES) made by malting two combination types include citric acid-glucose; choline chloride-sorbitol; malic acid-glucose; and lactic acid-sucrose. The extraction process was carried out using microwave-assisted extraction (MAE), and the determination of TPC was analyzed using Folin-Ciocalteau’s reagent and measured with a spectrophotometer type including 246.70 mg GAE/g sample (citric acid-glucose), 227.33 mg GAE/g sample (malic acid-glucose), 222.26 mg GAE/g sample (lactic acid-sucrose), and 219.02 mg GAE/g sample (choline chloride-sorbitol). According to the results, the NADES-MAE method shows differences in TPC based on the NADES types.