AKTIVITAS ANTIFUNGI FRAKSI ETILASETAT AKAR SINGAWALANG (PETIVERIA ALLIACEA L.) TERHADAP ASPERGILLUS NIGER

Abstract

Aspergillus niger is a mold that can infect respiratory tract in certain condition. Azoles are used to solve this infection. Drug development on antifungal drugs still continued, one of the resorce is from plant. A plant that widely studied as antifungi is singawalang (Petiveria alliacea L.). Activity of ethanol extract and fraction of singawalang roots on Aspergillus niger tested by microdilution broth method appropriate to Clinical and Laboratory Standard Institute (CLSI) standard. Microdilution test results showed that Singawalang roots extract has antifungal activity against Aspergillus niger with Minimum Inhibition Concentration (MIC) 32 μg/mL and Minimum Fungicidal Concentration (MFC) 1048 μg/mL. Fraction that has high activity against Aspergillus niger was ethylacetate fraction of Singawalang roots with MIC 128 µg/ml dan MFC 512 μg/mL. The higher activity of the extract than the fraction was predicted as the impact of multiple compounds that have synergic activity. The growth profile of Aspergillus niger showed unconstant result and tends to descend. However, further research needed to ensure this effect. Keywords: antifungal, microdilution, singawalang (Petiveria alliacea L.), Aspergillus niger