SOPIALENA-PROSIDING-ICTAFF3

Abstract

The banana plant (Musa sp.) is a horticultural commodity that has several advantages, including productivity, nutritional value, high genetic diversity, adaptability to a wide ecosystem, low production costs, and has been widely accepted by the community. The rhizosphere is the layer of soil that covers the surface of plant roots which is still influenced by root activity. The thickness and thinness of the rhizosphere layer is different for each plant. The rhizosphere is an excellent habitat for bacterial growth; therefore, plant roots provide various organic materials which generally become a place for microbial growth. The research was carried out at the Pest and Plant Disease Laboratory, Faculty of Agriculture, Universitas Mulawarman. This research aims to identify bacteria in the rhizosphere area of Kepok Banana, Rutai Banana and Wild Banana and to identify bacterial genera that have the potential to become PGPR. This research method is in the form of qualitative exploratory research, namely studying based on identification to then produce information that can be utilized. The research results obtained 32 isolates of rhizosphere bacteria from kepok bananas, 4 isolates of rhizosphere bacteria from rutai bananas and 4 isolates from forest bananas. Macroscopic morphological identification obtained varied colony shapes, elevations and colony edges in the three banana rhizosphere samples. After microscopic identification using gram staining of Kepok bananas, 12-gram positive isolates and 20 gram negative isolates were obtained, with 5 isolates in the form of bacilli and 27 isolates in the form of cocci. Forest banana rhizosphere bacteria 4 isolates were gram negative; 3 isolates were cocci and 1 isolate was bacillus. Forest banana rhizosphere bacteria 4-gram negative isolates, all in the form of cocci.