Antioxidant Assay Of Dayak Onion (Eleutherine palmifolia) Via DPPH (1,1-Difenil-2-Pikrilhidrazil) And BSLT Test For Its Active Fraction

Abstract

Dayak Onion (Eleutherine palmifolia) is a widely available plant in Kalimantan which has never been optimally analyzed for its significant. The aim of this research was to determine the active fraction of Dayak Onion (Eleutherine palmifolia) which possesses antioxidant activity. Antioxidant testing was carried out quantitatively with 5 test preparations namely: total ethanol extract, n-hexane fraction, chloroform fraction, ethyl acetate fraction, and ethanol fraction, were carried out using UV-Visible spectrophotometry through DPPH reagent to obtain IC50 Inhibition concentration. The test data results were processed with descriptive analysis. Furthermore, active fraction selected Brine Shrimp Lethality Test (BSLT) was carried out to determine the value of lethal concentration LC50 on shrimp larvae to see the toxicity category. The results showed that the chloroform fraction was an active fraction which had an IC50 of 20.29 ppm (very high category) which was active fraction h. BSLT Test Chloroform fraction has an LC50 value of 527.229 (medium category). In conclusion, Dayak Onion (Eleutherinepalmifolia) which can be widely found in East Kalimantan, Indonesia, is a powerful plant to be an alternative of antioxidant sources. Keywords: Antioxidant Assay, Active Fraction, Eleutherine palmifolia, DPPH, BSLT Test