Isolation and identification of potential lactic acid bacteria as probiotics from the intestines of repang fish (Puntioplites waandersi)

Abstract

Abstract. The present study aimed to isolate and characterize lactic acid bacteria (LAB) from the intestines of repang fish (Puntioplites waandersi), which have a potential as probiotic bacteria in vitro. The sample consisted of ten P. waandersi with a length of about 15.39±0.65 cm and a weight of about 40.04±6.24 g, taken from fishermen in the Mahakam River, Kutai Kartanegara Regency, East Kalimantan Province, Indonesia. The tests carried out included the isolation of LAB, characterization of isolates, antibacterial activity against pathogenic bacteria (Aeromonas hydrophila, Pseudomonas sp. and Edwardsiella ictaluri), antibiotic sensitivity, tolerance to several media and enzymatic activity. Five isolates of LAB have been isolated: Enterococcus sp. (R1 and R2), Lactobacillus sp. (R3), and Lactococcus sp. (R4 and R5). The five LAB had strong antibacterial activity against pathogens, some were resistant to antibiotics and had a tolerance to bile salts, salinity and temperature, and exhibited amylolytic, proteolytic, and lipolytic activities, so these LAB have potential as probiotics