HYDROCHALCONE COMPOUNDS FROM INDONESIAN MEDICINAL PLANT, “SIRIH HUTAN”, Piper aduncum (Piperaceae)

Abstract

Isolation, purification and identification of two hydrochalcone compounds from Indonesian medicinal plant “Sirihhutan”, Piper aduncum (Piperaceae) had been done. Isolation and purification of ethylacetate extract subjected to column chromatography (SiO2; (i).n-hexane-ethylacetate = 10:1 ~ 1 : 1; ethylacetate (ii).n-hexaneethylacetate = 8: 1 gave two pure compounds. Based on infra red, 1D & 2D-NMR, mass spectral data and comparison chemical shift of protons and carbons, the isolated compounds are 2’,6’-dihydroxy-4’-methoxy dihydrochalcone and 2’,6’,4-trihydroxy-4’-methoxy dihydrochalcone with free radical scavenging inhibitionvalues are 21.77 % and 90.1 % respectively.