Ligan Activity of Antiaris toxicaria Lesch. and the Role of Toxicarioside in Crude Extract: In Silico, In Vitro, and In Vivo Approaches

Abstract

Antiaris toxicaria Lesch is one of the endemic plant to Kalimantan Island and some tropical countries in South East Asia region. Traditionally, the sap of plant was used as poison and attached in traditional weapon. The crude extract of the sap plant contain toxic material called Toxicarioside. The aims of the research are to identify the toxicity of the crude extract which are derived from heating and methanol extraction process to Rattus norvegicus mortality and examine ligan Toxicarioside activity using in silico method. Extract toxicity was analyzed using percentages of probit analysis of the R. Norvegicus and a toxicity indicators. In silico analysis on the value of energy released and inhibition constanta of some molecule resulting the first confirmation of 2ZKD receptors has higher value -9.12 kcal/mol. Compared to the other nine confirmation, the value of inhibition constanta was the lowest 0.21 µM. Compared to the other nine conformation, receptor 3F8J, the minimum free energy which are able to release was about -6.26 kcal/mol and it has lowest inhibition constanta (25.89 µM). Crude extract of A. toxicaria from heating process has probit value =0.754x - 0.354 and LD50 = 1.017 or 10,339 mg while the extract derived from Methanol extraction has probit value =0.799x - 0.642 and LD50= 1.267 or 18.493 mg. These extract is the very toxic materials and has potentiality as bio-rodenticides. Keywords - Antiaris toxicaria, bio-rodenticides, Toxicarioside