AKTIVITAS EKSTRAK ETANOL DAN FRAKSI AKAR SINGAWALANG (Petiveria alliacea L. ) TERHADAP JAMUR PENYEBAB KETOMBE DENGAN METODE BROTH MICRODILUTION

Abstract

Dandruff was an anomaly of scalp caused by abnormal growth of Pityrosporum ovale. Ketoconazole and sulfuric compounds known as antifungal, include antifungal against Pityrosporum ovale. One of medicinal plant that has polysulfide compounds was Singawalang (Petiveria alliacea L.). Activity of ethanol extract and fraction of singawalang roots tested using microdilution broth method appropriate to Clinical and Laboratory Standard Institute (CLSI) standard, then growth profiles determined by colony count. Microdilution test results showed that Singawalang roots extract has antifungal activity against Pityrosporum ovale with Minimum Inhibition Concentration (MIC) 16 μg/mL and Minimum Fungicidal Concentration (MFC) 64 μg/mL. Fraction that has highest activity against Pityrosporum ovale was n-hexane fraction of Singawalang roots with MIC 16 µg/ml dan MFC 128 μg/mL. The higher activity of the extract predicted that there were some polysulfide compounds have synergic activity.

Key words : singalawang roots, polysulfide, Pityrosporum ovale