ANALISIS FITOKIMIA, TOKSISITAS DAN ANTIOKSIDAN EKSTRAK SERBUK SARI (Bee pollen) LEBAH Trigona spp.

Abstract

ABSTRACT Phytochemical analysis, toxicity test, the antioxidant activity and secondary metabolites of bee pollen a hive products Trigona spp. has been investigated. Bee pollen samples extracted with ethanol by masseration method with using a rotary evaporator. Ethanol obtained crude extract was fractionated by ethanol and ethyl acetate. Secondary metabolites of bee pollen with phytochemical analysis showed that the ethanol extract contains alkaloids and phenolic rough. The ethanol extract fraction containing alkaloids. Ethyl acetate extract fractions contain alkaloids and phenolic. In a test toxicity of an increase in mortality of larvae shrimp (Artemia salina L.) and then processed using SAS Probit Analysis to determine the lethal concentration 50% (Lc50). The results were obtained data shows that the most active fractions is extract ethanol with value is 249.6079 ppm. Test results of antioxidant activity by DPPH method using spectrophotometry showed that 50% inhibitory concentration (Ic50) is obtained in the crude extract was 104.84 ppm Ethanol, ethanol fraction value is 172.67 ppm, 75.99 ppm of ethyl acetate fraction and vitamin C is 10.79 ppm, this suggests that the most active fraction is ethyl acetate with Ic50 is 75.99 ppm.