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http://repository.unmul.ac.id/handle/123456789/17194| Title: | Uji Toksisitas Akut Larutan Temulawak (Curcuma xanthorrhiza) dan Pengaruhnya Terhadap Histopatologik Insang dan Ginjal Ikan Mas (Cyprinus carpio) |
| Other Titles: | Acute Toxicity Test of Java Ginger (Curcuma xanthorrhiza) Solution and The Effect to The Gill and Kidney Histopathological Change of Common carp (Cyprinus carpio) |
| Authors: | Sukarti, Komsanah |
| Keywords: | temulawak, toxicity, histopathology, gills and kidneys, carp |
| Issue Date: | Oct-2021 |
| Publisher: | AQUAWARMAN JURNAL SAINS DAN TEKNOLOGI AKUAKULTUR |
| Series/Report no.: | Vol.7 (2);89-96. |
| Abstract: | The aimed of this research were to determine the level of toxicity of Java ginger (Curcuma zanthorrhiza ) solution based on 96h-LC50 value, to analysed the histopathological of gill and kidney, swimming pattern and respiratory frequency of common carp (Cyprinus carpio) who were exposed the Java ginger solution for 96 hours. This research was conducted since September 2019 until June 2020 at Aquatic Toxicology Laboratory, Faculty of Fisheries and Marine Science Mulawarman University. Toxicity test consists of two stages, namely Preliminary test to determine lower threshold value (48h-LC0) was 100 mg.l-1 and upper threshold value concentration (24h-LC100) was 1000 mg.l-1. And further testing to determine 96h-LC50 with 6 test concentrations, namely 0 mg.l-1, 194 mg.l-1, 241 mg.l-1, 299 mg.l-1, 457 mg.l-1, and 696 mg.l-1. Each concentration with 3 replications. Analysis of Probability Unit was used to determine of 96h-LC50 value, histopathological changes of gill and kidney descriptively , swimming pattern and respiratory frequency with tabulation and descriptively . The result of this research showed that the 96h-LC50 value was 234.43 mg.l-1 which mean that concentration could caused 50% of the test animal’s mortality within 96 hours, and the criterion was moderately toxic. |
| Description: | Berdasarkan penelitian Uji Toksisitas Akut Larutan Temulawak (Curcuma xanthorriza) dan Pengaruhnya Terhadap Histopatologik Insang dan Ginjal Ikan Mas (Cyprinus carpio) dapat disimpulkan sebagai berikut: 1. Nilai LC50- 96 jam dari larutan temulawak terhadap ikan Mas sebesar 234,43 mg/L termasuk dalam tingkat toksik sedang menurut USEPA (1988), yang berarti pada konsentrasi 234,43 mg/L membunuh 50% total ikan serta mengakibatkan kerusakan histopatologis. 2. Perlakuan uji histopatologi insang pada perlakuan P1 (194 mg/L), P2 (241 mg/L), dan P3 (299 mg/L) mengalami kerusakan histologis insang tingkat 2 ditandai dengan terjadinya hiperopasia. Perlakuan P5 (696 mg/L) mengalami kerusakan ditunjukan dengan terjadinya fusi pada lamella sekunder yang merupakan ciri dari kerusakan tingkat 3 (Ikan mati). 3. Uji histopatologi ginjal pada perlakuan P1 (194 mg/L) kerusakan pada histologis ginjal berupa Inflammasi dan Nekrosis. Pada perlakuan P2 (241 mg/L), P3 (299 mg/L), dan P5 (696 mg/L) mengalami kerusakan yang tidak jauh berbeda berupa Inflammasi, Nekrosis, dan Odema. Pada perlakuan P3 (299 mg/L) dan P5 (696 mg/L) terjadi pelebaran bowman space dikarenakan mengkerutnya glomerulus. 4. Perbedaan pola renang ikan terlihat dari konsentrasi rendah ke konsentrasi yang lebih tinggi dan juga ferkuensi pernafasan ikan juga meningkat dari konsentrasi rendah ke konsentrasi yang lebih tinggi. |
| URI: | http://repository.unmul.ac.id/handle/123456789/17194 |
| ISSN: | 2460-9226 |
| Appears in Collections: | A - Fishery and Marine Sciences |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 12. 89-96. Romy NREP, Sulistyawati, K. Sukarti.pdf | 3.5 MB | Adobe PDF | View/Open |
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