PEMBUATAN ETANOL DARI SAGU SECARA FERMENTASI MENGGUNAKAN Saccharomyces cerevisiae DAN GELATIN SEBAGAI SUMBER NUTRISI BAGI MIKROBA

Abstract

ABSTRACTEthanol from sago flour through fermentation using Saccharomyces cerevisiae and gelatin as nutrition for microbe have been done. The making of ethanol through processes of hydrolysis, fermentation,distillation and analysis of ethanol concentration using Gas Chromatography instrument. The hydrolysis process of sago flour are used two steps which is liquification and saccharification using alpha-amylase and glucoamylase which will convert the starch into glucose. The fermentation process of glucose are done with time variation 5, 6, 7 and 8 days to the concentration variationof gelatin addition which is 0.5%, 1% and 1.5% (w/v) as nutrition source for microbe. The obtained ethanol from fermentationprocess are purified through distillation. Furthermore, analysis of ethanol concentration are used Gas Chromatography. The analysis results are showed the highest ethanol concentration obtained in 7 days with gelatin addition 1.5% are 79.50%