Analisis Bioautografi Dengan Kromatografi Lapis Tipis Pada Ekstrak Etanol Daun Caesalpinia sumatrana Roxb. Terhadap Bakteri Penyebab Infeksi Nosokomial

Abstract

Nosocomial infections are a major health problem in Indonesia; this is mainly due to their resistance to antibiotics. Based on this, the quest for new sources of natural based antimicrobial drugs is necessary. This study was conducted to test the potential of Caesalpinia sumatrana Roxb. leaves extract which is traditionally used as a medicine by Dayak tribe for wounds and skin infection. Bioautography analysis of plant extracts done by thin layer chromatography (TLC) against Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA). Results of research on extracts of C. sumatrana discovered containing sugar compounds which have potential antimicrobial activity against all bacteria; alkaloid compounds which have potential antimicrobial activity against S. aureus and MRSA; terpene-steroid compounds which have potential antimicrobial activity against P. mirabilis, S. aureus and MRSA; and flavonoid compounds which have potential antimicrobial activity against S. aureus. The conclusion of this study is the secondary metabolites of C. sumatrana leaves extract can inhibit the growth of bacteria that cause nosocomial infections.