Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus)

Abstract

Jintan hitam (Nigella sativa) has been widely used in traditional medicine to treat several diseases, including gastric ulcer. However, there is no scientific data demonstrating anti-ulcerogenic activity conferred by the use of jintan hitam. The present study aimed to evaluate the anti-ulcer properties of oil extract from jintan hitam. In the gastric ulcer induced by administration of 80% ethanol model, the observation made for ulcer score and index, level of ulcer erosion, the total amount of polymorphonuclear cells and morphological change of 80% ethanol-induced gastric ulcer of mice. The research was conducted on the laboratory in Faculty of Medicine Universitas Hasanuddin from April to September 2015, with post test only experimental group design. There were 40 mice used in this research, divided into five groups, each group consisted of 8 mice: negative control group, positive control group, and three different doses of oil extract (0.1, 0.2 and 0.3 ml per 20g bw of mice). Observation made for 4 and 7 days. The oil extract of jintan hitam showed significant differences on doses of 0.1 ml per 20g bw of mice given in 4 days, with p = 0.034 (p<0.05) based on score and index of gastric ulcer.