Alpha-Mangostin Effect on Inhibition Development Stadium and Globin Accumulation Against Plasmodium falciparum

Abstract

a-Mangostin is a widely reported group of Xanthone compounds from the Clusiaceae family of 40 genera and over 1000 species spread across the tropics and subtropics area. The Objective for determine effect of a-mangostin from Garcinia tetrandra Pierre stem bark against development stadium inhibition and globin accumulation of Plasmodium falciparum. Inhibition stadium development assay used based on the Rosenthal method. Plasmodium falciparum parasitic globin accumulation assay. Globin accumulation assay used the highest concentration of in vitro antimalarial test using SDS-PAGE with positive control E-64 and a-mangostin were incubated together with the malaria parasite during 24 h. Result of Inhibition stadium development of Plasmodium falciparum against a-mangostin show inhibition from development stadium of the malaria parasite Plasmodium falciparum, Electrophoresis show globin accumulation from electrophoresis followed by staining using Coomassie brilliant blue. a-mangostin showed inhibition the growth of malaria parasite Plasmodium falciparum with dose 10 ?g/ml is expected to occur a large accumulation of globin, which can be viewed both morphologically and by the method of SDS-PAGE.