Aktivitas Antibakterial Ekstrak Daun Temu kunci (Kaempferia pandurata) terhadap bakteri A. hydrophila

Abstract

This study aims to assess the leaf extract of temu kunci (Kaempferia pandurata), as an antibacterial of A. hydrophila. The experiment was conducted at the Laboratory of Aquatic Microbiology, Faculty of Fisheries and Marine Sciences, University Mulawarman, which began in January to February 2014. Concentration of leaf extracts are used in the temu kunci inhibition test order for Agar Disc Diffusion method (ADD) on the growth of bacteria A. hydrophila is 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm, 900 ppm, 1000 ppm, 1500 ppm, 2000 ppm, 2500 ppm, 3000 ppm, 3500 ppm, 4000 ppm, 4500 ppm, 5000 ppm, 5500 ppm and 6000 ppm.As a positive control is antibiotic Amoxicillin and fisiogis solution as a negative control. Ekstrak dripped on paper discs, placed on the media that has overgrown BHIA A. hydrophila bacteria. Then further tested with a bacterial culture test with the extract, the bakteria count using the Total Plate Count and Spektrofotomter.Concentration was used the best ADD 600, 700, 800, 900 ppm, which the best inhibition zone ranged 10,5-13 mm. The results showed leaf extracts of K. pandurata are antibacterial against A. hydrophila. In ADD the best leaf extract concentrations is 900 ppm, while in the bakterial culture countextract concentration is 600 ppm.