Efikasi Vaksin Monovalen A. hydrophila pada budidaya ikan nila (Oreochromis niloticus)

Abstract

This study aims to determine the efficacy of monovalent vaccine of A. hydrophila to prevent infection A. hydrophila and Pseudomonas sp. in tilapia. The vaccine made from whole cells of A. hydrophila bacteria and inactivated with formalin 3%. Vaccination trial was done through intraperitonial injection into fish having mean weight of 15-20 g. Control negative tilapia were injected with NaCl fisiologis. Fish were vaccinated with whole cell and challenged by IP injection with 1010 colony-forming units (CFU)/fish of A. hydrophila and Pseudomonas sp. Tilapia vaccinated with monovalent A. hydrophila had a Relative Percent Survival (RPS) of 74% (IP challenged with A. hydrophila) and 100% (IP challenged with Pseudomonas sp.). This indicates that A. hydrophila monovalent vaccine could prevent bacterial infections A. hydrophila and Pseudmonas sp.