| dc.description.abstract | Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americanabulb extract and its characterization.Nusantara Bioscience 13:247-254.The plant-based biosynthesis of nanoparticles has gained increasingmomentum due to being lower in cost and eco-friendly. This study aimed to biosynthesize nanoparticles from the bulb ethanolic extract of Eleutherine americana(Aubl.) Merr. ex K.Heyne (Ea-AgNPs), then characterize Ea-AgNPs and determine their phytochemical content and antioxidant capacity. The Ea-AgNPs were synthesized using ethanolic extract of E. americanabulb along with various concentrations of AgNO3(0.5-4 mM). The Ea-AgNPs were then characterized using UV-VIS spectroscopic, Scanning Electron Microscopy/Energy Dispersive X-ray(SEM/EDX), Transmission Electron Microscopy (TEM), X-ray Powder Diffractometry (XRD), and Fourier-Transform Infrared Spectroscopy (FTIR)techniques. The results indicated that E. americanacan be used to reduce AgNO3to synthesize Ea-AgNPs, indicated by color change, and had optimum UV/VIS spectra at 400nm. The FTIR analysis found that Ea-AgNPs showed peaks at 2919, 2850, 1586, and 1031 cm1, containing several important biocompounds. Additionally, the XRD results found an amorphous Ea-AgNP peak with maximum intensity and proportion of silver occurring at 24 Theta. The particle size distribution curve of Ea-AgNPs showed a size of 105nm. Furthermore, SEM/EDX analysis revealed an optical absorption characteristic peak at 3 keV. The EDX examination revealed three signals: a strong signal from the C atom (70.99 %), an O atom (28.95 %), and Ag atom (0.06 %). The TEM imaging also showed the characteristics of Ea-AgNPs. Some phytochemicals such as flavonoids, tannins, alkaloids, and saponins were found in Ea-AgNPs, withIC50values of 45.30 ppm | en_US |
