In vitro evaluation of the vasodilatory activity of ethanol extracts of Eleutherine bulbosa bulbs and leaves
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Date
2021-05-05Author
Fikriah, Ika
Ismail, Sjarif
Kosala, Khemasili
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The aim of this study was to examine and compare the vasodilatory activities of ethanol extracts of Eleutherine
bulbosa bulbs (EBBs) and leaves (EBLs) on isolated aortas from Wistar rats, with and without endothelium. EBBs and
leaves EBLs were washed with clean water and chopped and dried in a 60°C drying cabinet. Extraction was carried
out by maceration using an ethanol solvent. The collected filtrate was concentrated using a vacuum evaporator, and
the concentrated liquid was dried further in a 60°C oven. By carrying out relevant bioassays, the ethanol extracts
of EBBs and leaves EBLs were evaluated using Wistar rat aortic rings, with and without endothelium in Krebs–
Henseleit solution. Aortic rings were precontracted with phenylephrine solution, and after reaching the plateaued peak
contraction, a single dose of the extract solution was administered, and the contractility was measured. The percentage
of aortic dilation was presented as mean ± SD and statistically analyzed using the t-test, deemed significantly different
if the p-value was < 0.05. The results showed that E. bulbosa leaf extracts induced vasodilation of aortic rings without
endothelium, which was stronger than E. bulbosa bulb EBB extracts, indicating the vasodilatory activity through the
endothelium-independent vascular smooth muscle.
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- J - Pharmacy [579]