| dc.description.abstract | Applying green chemistry principles in the exploration of an active secondary metabolite from natural
products has continued to experience a significant increase in the last decade, mainly natural deep
eutectic solvent (NADES) usage. Mitragyna speciosa (Korth.) Havil (Rubiaceae family) is a native of East
Kalimantan, which is traditionally used as an herb to treat headaches and inflammation by drinking boiled
water a few leaves. On the other hand, this plant has an addictive effect. This study aims to determine
the single factor effect of NADES citric acid–glucose-based microwave-assisted extraction (MAE) to
obtain the yield of total polyphenols content (TPC) from M. speciosa leaves. Dried powder of M. speciosa
leaves was extracted using NADES citric acid-glucose based MAE method with some different conditions
such as NADES (citric acid-glucose) ratio (4:1, 5:1, 6:1 g/g), solid-liquid ratio (10:1, 15:1, 20:1 mL/g),
microwave power (90, 270, 450 Watts), and extraction time (15, 20, 25 min). The determination of TPC
was performed using Folin-Ciocalteau reagent and gallic acid as a standard with different concentration
levels. The absorbance measured using spectrophotometer UV-VIS at 746 nm to obtain a regression
formula of Y = 0.0022X–0.00095, where R2 = 0.9977. Based on the result, the best of single factor
condition effect was obtained the TPC value including NADES ratio of 248.69 mg GAE/g sample (5:1 g/g
citric acid-glucose), a solid-liquid ratio of 146.93 mg GAE/g sample (15:1 mL/g solvent-sample), microwave
power of 192.20 mg GAE/g sample (270 Watts), and extraction time of 358.59 mg GAE/g sample (15 min).
The single factor effect of NADES citric acid-glucose-based MAE shows a difference in TPC value based
on various conditions of this method. | en_US |
