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Solubilization technique of membrane-bound enzyme: Sialidase from horse liver
(ASEAN Biochemistry Seminar, Enzymes: Industrial and Medical Prospects, Surabaya, February 6-7, 2006., 2006)
Two different buffers containing salt were determined to bring sialidase in 100,000 g supernatant,
while some detergents like cholic acid, taurocholic acid and Triton X-100 were tried as solubilizing agents. Acetate buffer ...
Catabolism of 4-O-Acetylated Sialic Acid
(ASEAN Biochemistry Seminar, Enzymes: Industrial and Medical Prospects, Surabaya, February 6-7, 2006., 2006)
“Different” sialidases from partially purification of two kinds of horse liver was studied to find out
the catabolism of 4-O-acetylated N-acetylneuraminic acid (Neu4,5Ac2) with guinea pig serum as substrate. Using ...