Enhanced Extraction of Total Polyphenols Content from Mitragyna Speciosa (Korth.) Havil Leaves using the Natural Deep Eutectic Solvent-based Microwave-assisted Extraction Method
Date
2019Author
Ahmad, Islamudin
Cahyo P, Wisnu
Nur, Yuspian
Irawan, Lulu
Yusniah, Andi
Puji R, Bakti
Hidayati, Ramila
Nurlinda, Hesti
Herman, Herman
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Exploration of natural products is highly dependent on separation techniques, mainly solvent selection, one
of which is using the green chemistry principle approach. Mitragyna speciosa (Koth.) Havil is an endemic
plant of East Kalimantan which traditionally used for the treatment of various diseases. On the other hand,
this plant has an addictive effect. The study aims to determine the impact of using natural deep eutectic
solvent-based microwave-assisted extraction (NADES-MAE) on total polyphenol content (TPC) extraction
from M. speciosa. Natural deep eutectic solvent (NADES) made by malting two combination types include
citric acid-glucose; choline chloride-sorbitol; malic acid-glucose; and lactic acid-sucrose. The extraction
process was carried out using microwave-assisted extraction (MAE), and the determination of TPC was
analyzed using Folin-Ciocalteau’s reagent and measured with a spectrophotometer type including 246.70 mg
GAE/g sample (citric acid-glucose), 227.33 mg GAE/g sample (malic acid-glucose), 222.26 mg GAE/g
sample (lactic acid-sucrose), and 219.02 mg GAE/g sample (choline chloride-sorbitol). According to the
results, the NADES-MAE method shows differences in TPC based on the NADES types.
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- J - Pharmacy [579]