Repairing Effects of Aqueous Extract of Kalanchoe pinnata (Lmk) Pers. on Lupus Nephritis Mice
Date
2018-05-01Author
Indriyanti, Niken
Garmana, Afrillia Nuryanti
Setiawan, Finna
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Kalanchoe pinnata (Lmk) Pers (KP) has an immunosuppressive effect on delayed-type hypersensitivity test. Based on it, this research aimed to determine the repairing effects of aqueous extract of KP on lupus nephritis mice and identified its active compound. The KP extract
profile was determined using UPLC-QTOF-MS/MS instrument. We examined six mice groups
consisting of three curative treatment groups, one standard group receiving prednisone, one
preventive group receiving KP extract, and one healthy (healthy and untreated) group. At the
end of the experiment, we measured the proteinuria and renal histology parameters. To
recognize the active compound in the KP profile, we performed in silico assays for the flavonoid
compounds to bind to the glucocorticoid receptor. We played in silico tests for the flavonoid
compounds to identify the active compound in the KP profile. We found the repairing effect
of KP was detected in the kidney, demonstrated by its low proteinuria level and its better
tissue structure. In the curative group, the urine protein level and its glomerular inflammation
decreased. In the preventive group, the aqueous extract of KP could prevent lupus nephritis
manifestations in the kidney. Bryophyllin A is the most active compound of the KP. However,
further research is needed to understand the mechanism involved. We conclude, the aqueous
extract, especially its bryophyllin A, have beneficial effects in repairing the function and tissue
structure of lupus manifestations in mice kidney
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