Metabolite Profling Analysis of Conventional and Non-Conventional Extraction Methods on Secondary Metabolite from Peperomia pellucida (L.) Kunth using UPLC-QToF-MS/MS System

Abstract

Objective: The purpose of this study was to observe the difference of extraction method (both conventional and non-conventional) based on metabolite profle using UPLC-QToF-MS/MS system from Peperomia pellucida (L.) Kunth. Methods: Dried samples were extracted using the conventional maceration method and the optimum ofonic liquid-based microwave-assisted extraction (IL-MAE) methods.Metabolite profling was performed using UPLC-QToF-MS/MS system with some modifcations adjusted to the instrument condition. The data was analyzed using Masslynx 4.1 software. Results: based on the results, there were differences on metabolite profling from both conventional and non-conventional extraction methods that was extraction method using organic solvent and ionic liquid solvent ([BMIM]BF 4 at the optimum condition.The extract obtained using IL-MAE method had a peak depth with a well-separated Rt (retention time) value ranging from 0.5 to 7.5 min which means that the extracted compound was from polar to nonpolar properties. The extract obtained using maceration method, the peak spread on a separate Rt ranges from 2.5 to 7.5 min. Also, both extracts obtained have different area under curve (AUC) values with amount total of 22285 (IL-MAE) and 12679 (maceration), respectively, and showed the IL-MAE twice as large as that of the maceration. Conclusion: based on the results, conventional and non-conventional extraction method showed differences in metabolite profiling based on Rt value and mass spectrum m/z of each peak.