Pengaruh Pemberian Ekstrak Phyllanthus niruri Linn Terhadap Infiltrasi Limfosit dan Ekspresi Perforin pada Kanker Kolon Tikus Sprague-Dawley

Abstract

The effect of Phyllanthus niruri Linn extract on lymphocytes infiltration and perforin expression in colon cancer of Sprague-Dawley Rat Background: Colon cancer treatment currently involves immunotherapy that aims to improve the quality of life and survival of patients. Phyllanthus niruri Linn (P. niruri L) may act as an immunomodulator, but its potency in antitumor immune responses has not been revealed. The study was conducted to evaluate the differences between the immunological status of rats suffering colon cancer which were not given to those given the extract of P. niruri L. Methods: The study was randomized posttest-only control group design. Samples were 30 Sprague-Dawley male rats, bodyweight 170-220 gr which induced by 1.2 DMH 30 mg/kgBW subcutaneously. On the weeks 9, 11 and 13, four induced rats each week were sacrified to detect the development of colon cancer. On the weeks of 13th all of 4 rats were developed colon cancer, so the induction were stopped. The rest of 18 induced rats were randomly into two groups: without P. niruri L or positive control (K+)=9 rats and given P. niruri L extract 13.5 mg/kg orally or X group=9 rats. After 19th week all of rats were then terminated and tumor lesion of colon were processed hystophatologically. The tissues of colon cancer were stained by H&E for evaluate the lymphocytes infiltration and immunohistochemistry monoclonal antibody anti-perforin for perforin expression. Non pairs t-test was used with considered significant if p<0.05. Results: The mean of lymphocytes infiltration of the group X was 401.89±70.19, it was higher compared to K+ 191.89±50.68 (p=0.000). The mean percentage of perforin expression of group X was 39.00±1.80%, it was higher compared to K+ 23.00± 3.00% (p=0.000). Conclusion: The extract of P. niruri L increases immunologic status through mechanism of lymphocytes infiltration and perforin expression elevation of colon cancer in animal mode.