EVALUATION OF SYNERGISTIC EFFECT OF KAEMPFERIA GALANGA L. RHIZOME EXTRACTS WITH ANTIBIOTICS AGAINST BACTERIAL PATHOGENS

Abstract

The aromatic ginger rhizome (Kaempferia galanga L.) is a member of Zingiberaceae family, has been known to have an antibacterial effect is used for everyday cooking spices, but the synergistic effect on broad spectrum antibiotics was unknown. This research was conducted to find out the synergistic effect is on the antibiotics or the synergistic effect of the combination of the extract and antibiotic against gram positive and negative bacterial pathogens in vitro. KGR was taken from farmers in Samarinda City, East Kalimantan Province, Indonesia. Extract was obtained by maceration with ethanol solvent. Antibacterial activity test of KGR ethanol extract and antibiotic using Mueller-Hinton agar, Kirby-Bauer disc diffusion method against Staphylococcus aureus and Escherichia coli. Results were expressed in percentage increase of the inhibition zone. Statistical test with t-test, significantly different if p<0.05. The combination of each antibiotics and KGR ethanol extract showed synergistic effect by increasing the percentage of inhibition zone Conclusion: KGR ethanol extract showed synergistic effect on antibiotic ampicillin (E. coli), meropenem and cefuroxime in S. aureus and E. coli.