Characterization of Extracellular Proteins Produced By Aeromonas hydrophila Cultured At Different Conditions

Abstract

Esti Handayani Hardi, Gina Saptiani and Angela Mariana Lusiastuti. 2014. Characterization of Extracellular Proteins Produced By Aeromonas hydrophila Cultured At Different Conditions. International Conference of Aquaculture Indonesia 2014. Bacterial disease that is often found in tilapia fish cultivation among other groups of bacteria Aeromonas and Pseudomonas. In early 2011, found a tilapia in cages in Loa Kulu, Kutai Kartanegara in East Kalimantan death exceeds 60%, the observation known bacterial cause of death was Aeromonas sp. and Pseudomonas sp. both bacteria have different specific characteristics which influence to they pathogenicity. The aim of this research was to characterize extra cellular proteins produced by Aeromonas hydrophila cultured in different conditions. The bacterium was grown on TSA and in TSB media with temperature at 30oC and 40oC during 24, 48 and 72 hours. The ECP proteins were analyzed by SDS PAGE using the method described by Laemmli with silver nitrate stained and protein sequencing using mass spectrometry. A Protein of approximately 31 kDa was detected on the gel where the ECPs from the TSA cultured bacteria that has been incubated at 40oC ware analyzed. It is possible that this could be a toxic protease caused sickness and death to nila tilapia.