Antioxidant Activity from Lygodium microphyllum Aerial Parts

Abstract

Lygodium microphyllum is an invasive plant known as another name “Old World Climbing Fern” or considered a weed plant. Research on L. microphyllum related pharmacological activity is limited. This study aims to evaluate antioxidant activity from Methanol extract and Fractionation using n-hexane, Ethyl acetate and water solvent with a different polarity from L. microphyllum plant. Methods: Aerial part of L. microphyllum was macerated with methanol solvent, the extract obtained in fractionation using solvents with different polarity levels and was tested in invitro free radical using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The IC50 result was 49.328 ± 0.242 ppm for methanol extract, n-hexane extract was 32.041 ± 0.770 ppm, and ethyl acetate extract 8,732 ± 0.204 ppm and water extract 93.330 ± 1.931 ppm. The conclusion from this study reveals the results of extraction using ethyl acetate solvent showed the highest value antioxidant activity against DPPH.