Isolasi dan Identifikasi Steroid dari Ekstrak N-Heksana Daun Afrika (Vernonia amygdalina Del.)
Abstract
ABSTRACT : Isolation and identification of steroids from the n-hexane fraction of Vernonia amygdalina Del. was
carried out. Separation and purification were performed using flash chromatographic and gravity
chromatography. Based on the results of phytochemical tests supported by UV and FT-IR data analysis, the
isolate obtained was a steroid (sterol type). Toxicity test results using the Brine Shrimp Lethality Test (BSLT)
were known that the steroid has moderate toxicity with an LC50 value of 48.39 ppm.