Secondary Metabolites Compounds Profile by LC-MS/MS and Chemical Methods from Kolowe Stem Bark (Chydenanthus excelsus)

Abstract

Secondary metabolites's profiles from stem bark of Kolowe (C. excelsus) are steroidal saponin and glycoside's flavonoids, which consists of five kinds of steroidal saponins and eight types of flavonoids glycoside's compounds. Both these classes of compounds in the chemical identification and analysis on the results by LC-MS/MS. Pharmaceutical potential that has been revealed from a mixture of secondary metabolites compounds such are cytotoxic, antioxidants and antimicrobials activity. Information of cytotoxic activity during the test results with bio-indicators of the third instar larvae of A. salina, antioxidant activity with DPPH radical compounds test indicators, while the antimicrobial is a result of screening against 19 types of microbes (bacteria and fungi). Other potential methanol crude extracts from stem bark of Kolowe (C. excelsus) is the yield that is 8.97%, which is extracted by cold maceration method using methanol 96%.