Microelement Analysis In Edible Muscle Of Oreochromis Niloticus From Two Different Age Of Reclaimed Post Coal Mining Ponds East Kalimantan Using Sem-Edx
Abstract
Abstract : This study aims to evaluate the ultrastructure of the muscle and bioaccumulation of heavy metal elements in Oreochromis niloticus muscle
as a biomarker of environmental pollution in post-coal mining ponds. The fishes sample come from three different ponds, namely from the Control
pond,Senong pit (5 years old), and Goldstar pit (10 years old). Heavy metal elements in fish meat were analyzed using Scanning Electron Microscopy
combined with an Energy Dispersive tool X-ray (SEM-EDX). The results showed that the level of accumulation of heavy metals (K, Cu, Zn) in fish
samples with the highest percentage of metal elements was Cu between 1.48-1.74%; Zn between 0,80-1.17% and K between 1.58-2.43%. Histological
lesions in the muscle tissue of Tilapia, O.niloticus are in line with the accumulation of the metals. The levels of Cu, Cl, and K in muscle from Control are
lower in longer ponds, highest Zn percentage in old ponds. These results provide evidence that microanalysis of metal elements in tissue with SEM-EDX
and fish muscle ultrahistopathology as biomarkers can be used as an indicator of pollutant exposure in post-coal mine land waters. The findings of this
study are expected to be used as consideration in the aspect of diagnostic methods in monitoring fish health status and quality of fish products
originating from post-mining ponds.