Effect of Pulse Electromagnetic Field Exposure on the Expression of Lipo Protein Lipase (LPL) on the Differentiation of Mesenchymal Stem Cell
Abstract
Pulsed electromagnetic fields (PEMFs) have an important role in cell differentiation.
Previous study reported that PEMFs had positive and negative effect towards cell differentiation
that depends on their frequencies applied to the cells. Human adipose-derived stem cells (ASCs)
are mesenchymal stem cells that have an ability to differentiate into several types of cell
including adipocytes, chondrocytes and osteocytes. This study aimed to evaluate the effect of
human ASCs towards their adipogenic differentiation during PEMFs exposure. Human ASCs
were isolated from adipose tissue. The cells then cultured in specific medium of adipocyte that
induced ASCs differentiation along with PEMFs exposure. The maximum magnetic field used
is 2 mT with a frequency of 75 Hz. To confirm the effect of PEMFs exposure towards adipogenic
differentiation, mRNA expression of lipo protein lipase (LPL) was measured in mRNA
expression level. The results showed that ASCs cultured on adipogenic differentiation without
PEMFs exposure gradually increased LPL expression until day 14 of observation, while ASCs
with PEMFs exposure significantly decreased LPL expression from day 2 to day 14. Based on
the results, we concluded that PEMFs exposure can inhibit LPL expression that suppressed
adipogenic differentiation