Synthesis and Bioactivity of New Pyrazoline Derivative: N-carbamide-3-(2,4-dichlorophenyl)-5-(4-hydroxy-3- methoxyphenyl)pyrazoline

Abstract

Pyrazoline is an alkaloid class rarely found in nature. The pyrazoline possesses biological activities such as anti-inflammatory, antibacterial, antioxidant and antidiabetic. Pyrazoline has potential as candidate of new drug molecules in pharmacy. This study aims to synthesize new pyrazoline derivative and with the preliminary test through their toxicity and antibacterial activity to know its potential as a new drug candidate. The synthesis of N-carbamide-3-(2,4-dichlorophenyl)- 5-(4-hydroxy-3-methoxyphenyl)pyrazoline was conducted from 2',4'-dichloro-4-hydroxy-3-methoxy chalcone and semicarbazide through cyclization by refluxing for 8 hours. It was then characterized by TLC, UV-Vis, 1H-NMR, 13C-NMR and LC-Mass Spectroscopy. The toxicity test was carried out by Brine Shrimp Lethality Test (BSLT) and antibacterial activity screened by Agar Diffusion method against Staphylococcus aureus and Escherichia coli bacteria. Based on this study, the LC50 value was 96,96 ppm to Artemia Salina Leach which shows its potential as an anticancer agent. Antibacterial activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 was classified as moderated-strong inhibition.